Ansul Lokdarshi

Teaching Interests 

• Laboratory Techniques in Biotechnology
• Plant Physiology & Biotechnology
• Cell Biology
• Experimental Design and Biostatistics

 Research Interests 

• I am a plant molecular biochemist with broad interests in the principles of genetics, cell biology, and stress physiology, and 15+ years of experience in formulating, troubleshooting, and managing interdisciplinary projects. My research interests are in understanding the regulation of protein synthesis. In addition, I am interested in developing new technologies for plant phenotyping, setting intellectual property initiatives, and creating innovative, hands-on teaching tools that foster experiential, research-based student learning.

Selected Publications (Undergraduate or ^Graduate student author *Corresponding  or   †Co-corresponding author) 

1. ^Wynn M, Lokdarshi A*, ^Diaz DR. 2025. Activation of the Arabidopsis thaliana GCN2-eIF2α module by salicylic acid: Biochemical and phenotypic similarities between gcn2 mutant and wild type. microPublication Biology. 10.17912/micropub.biology.001771. https://micropublication.org/journals/biology/micropub-biology-001771
2. Lokdarshi*. Book Chapter - Chloroplast Translation Machinery. In: Burch-Smith, T.M. (eds) Chloroplast Gene Expression: Regulation, Stress Signaling and Biotechnology. Nucleic Acids and Molecular Biology, vol 37. Springer, Cham. https://doi.org/10.1007/978-3-031-70098-9_4. 
3. ^#Brylie A. Ritchie, Theodore A. Uyeno, ^Daniel Rincon F. Diaz, and Ansul Lokdarshi*. “A Novel Imaging Protocol for Investigating Arabidopsis thaliana Siliques and Seeds Using X-rays”. Bio Protoc. 2023, doi: 21769/BioProtoc.4839. 
4. Ansul Lokdarshi† and Albrecht G. von Arnim. “A miniature sucrose gradient for polysome profiling”.  (Bio-protocol 2023, doi: 21769/BioProtoc.4622). 
5. Ansul Lokdarshi*, Albrecht G von Arnim, ^#Teressa K Akuoko. “Modulation of GCN2 activity under excess light stress by osmoprotectants and amino acids”. Plant Signaling & Behavior 2022, https://doi.org/10.1080/15592324.2022.2115747. 
6. Ansul Lokdarshi†, Albrecht G von Arnim. “Emerging roles of the signaling network of the protein kinase GCN2 in the plant stress response”. Plant Science 2022, https://doi.org/10.1016/j.plantsci.2022.111280. 
7. G. Beamer, P. Routray, W.C. Choi, ^#M. Spangler, A. Lokdarshi, and D.M. Roberts. “Aquaporin family lactic acid channel NIP2;1 promotes plant survival under low oxygen stress in Arabidopsis”. Plant Physiology 2021, https://doi.org/10.1093/plphys/kiab196. 
8. Ansul Lokdarshi, Ju Guan, Ricardo A Urquidi Camacho, Sung Ki Cho, ^#Philip Morgan, ^#Madison Leonard, Masaki Shimono, Brad Day, Albrecht G von Arnim. “Light activates the translational regulatory GCN2 kinase via reactive oxygen species emanating from the chloroplast”. The Plant Cell 2020, https://doi.org/10.1105/tpc.19.00751. 
9. Ansul Lokdarshi, ^#Philip Morgan, ^#Michelle Franks, ^#Zoe Emert, ^#Catherine Emanuel, and Albrecht von Arnim. “Light dependent GCN2 activation under cold and salt stress is mediated by reactive oxygen species”. Plant Sciences 2020, https://doi.org/10.3389/fpls.2020.00431. 
10. Ansul Lokdarshi, Csaba Papdi, Aladár Pettkó-Szandtner, Stefan Dorokhov, Ben Scheres, Zoltan Magyar, Albrecht G. von Arnim, Laszlo Bogre and Beatrix M. Horvath. “EBP1 Regulates the Capacity for Protein Translation and Counteracts RBR to Maintain the Root Meristem”. Plant Physiology 2020, https://doi.org/10.1104/pp.19.00805. 
11. Ricardo A Urquidi Camacho, Ansul Lokdarshi (Co-first author), and Albrecht von Arnim. “Translational gene regulation in plants: A green new deal”. Wiley WIRE’s RNA 2020, https://doi.org/10.1002/wrna.1597. 
12. Ansul Lokdarshi, Craig Conner, Carlee McClintock, Tian Li and Daniel M. Roberts. 2016. “Arabidopsis CML38, a calcium sensor that localizes to ribonucleoprotein complexes under hypoxia stress”. Plant Physiology 2016, https://doi.org/10.1104/pp.15.01407.